Knockdown of GOLPH3 inhibited 5-FU-induced apoptosis in CRC cells. RKO (A) and LoVo (B) cells were transfected with GOLPH3 siRNA pool (siG-pool) or control siRNA (siControl), and then treated with 5-FU of 0 or 200 μM for 48 h. Apoptosis was detected by flow cytometry using Annexin-V-FITC and propidium iodide (PI) dual labelling. Data are presented as percentage of early and late apoptotic cells of total number of cells examined. All experiments were performed in triplicate. The values are mean ± SD, *P < 0.05.