Figure 7
Effect of Pyk2 inhibition by PP1 on tumor cell invasiveness after bevacizumab (Bev) treatment in vivo . (A) Rat C6 glioma xenografts treated with Bev alone or Bev plus PP1 were stained immunohistochemically for vimentin to show the invasion of tumor cells. In the Bev alone treated group, tumor cells had clearly invaded the surrounding normal brain. However, the necrotic areas (N) in the Bev plus PP1 treated tumors increased and the tumor cells invading the surrounding normal brain decreased. The right upper corner shows a higher magnification image (boxed inset). (B) Quantification of vimentin-positive cells invading the surrounding normal brain with Bev alone or Bev plus PP1 treatment. The number of individual cells crossing the solid tumor rim was counted in five fields. The invasive tumor cell number of the group treated with Bev alone were expressed as 100% (**p < 0.01, vs. the group treated with Bev alone).