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Table 1 Nucleotide sequences of oligonucleotide primers used for PCR and sequencing of KRAS

From: Detection of activated KRAS from cancer patient peripheral blood using a weighted enzymatic chip array

  

Sequences

PCR product (bp)

Gene

Exon

Forward primer (5′ → 3′)

Reverse primer (5′ → 3′)

 

KRAS

1

TAATACGACTCACTATAGGGAGATATGTTGAGGGCCCATCTCTC

TCCTAGGTCAGCGCAACCAAAT

131

KRAS

2

TAATACGACTCACTATAGGGTTCCTACAGGAAGCAAGTAG

CACAAAGAAAGCCCTCCCCA

148

Sequencing primer

CCCTATAGTGAGTCGTATTA

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