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Figure 5 | Journal of Translational Medicine

Figure 5

From: Sulfur compounds block MCP-1 production by Mycoplasma fermentans-infected macrophages through NF-κB inhibition

Figure 5

M.F. induction of MCP-1 through a TLR-mediated mechanism. U937 cells were infected with M.F. and treated with the following inhibitors: IKK Inhibitor VII (200nM) and TIRAP Inhibitor peptide (10 μM) to induce NF-κB inhibition (A), MyD88 Inhibitory Peptide (75 μM) to inhibit MyD88 homodimerization (B) and SB203580 (400 μM) to inhibit p38-MAP (A). The inhibitors were added daily. Supernatants were collected at 48 hours and tested for MCP-1 production by ELISA assay. MCP-1 production was normalized with the Calcein viability assay, as described in the Materials and Methods section. Bars denote the standard deviation. The p-values were calculated as unequal variance t-test of Mycoplasma infected cells relative to 243 media control (*p ≤ 0.05; **p ≤ 0.005), as unequal variance t-test of Mycoplasma infected cells treated with H2S donors relative to Mycoplasma infected cells (#p ≤ 0.05; ##p ≤ 0.005) and as unequal variance t-test of Mycoplasma infected cells treated with inhibitors relative to Mycoplasma infected cells treated with control inhibitors (+p ≤ 0.05; ++p ≤ 0.005; ns not significant). The histograms shown are representative of data from four different experiments.

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