Figure 3
Inhibition of MCP1 production by H 2 S in M.F. infected U937 cells and macrophages. A: U937 cells were infected with M.F., the two H2S donors (NaHS and GYY4137) were directly added to the media at the concentrations of 1 mM for NaHS and 0.1 mM for GYY4137 and the supernatants were collected 48 h and 72 h after the infection/treatments, and tested for MCP-1 production by ELISA. IL-6 was used as positive control for MCP-1 production. B: Macrophages were infected by M.F. and treated with H2S donors at the same concentrations (1 mM for NaHS and 0.1 mM for GYY4137) and the supernatants were tested by ELISA assay at 48 h and 72 h following infection and H2S treatments. MCP-1 production was normalized with the Calcein viability assay, as described in the Materials and Methods, in both experiments. Bars denote the standard deviation. The p-values were calculated as unequal variance t-test of Mycoplasma infected cells relative to 243 media control (*p ≤ 0.05; **p ≤ 0.005; ns not significant) and as unequal variance t-test of Mycoplasma infected cells treated with H2S donors relative to Mycoplasma infected cells (#p ≤ 0.05; ##p ≤ 0.005). The histograms shown are representative of data from five different experiments.