Skip to main content
Figure 1 | Journal of Translational Medicine

Figure 1

From: Sulfur compounds block MCP-1 production by Mycoplasma fermentans-infected macrophages through NF-κB inhibition

Figure 1

MCP-1 induction by M.F. in U937 cells and macrophages. A: U937 cells were infected with M.F. and the supernatants were collected at the following time points 24 h, 48 h, 72 h, and tested for MCP-1 production by ELISA. Levels of MCP-1 were indicated at each time point. B: RNA samples from U937 cells infected with M.F. were collected at the established time points (3 h, 6 h, 18 h and 24 h). MCP-1 expression was analyzed by real time quantitative RT-PCR as described in Materials and methods procedures. U937 cells treated with 243 M.F. growth media (v/V) were used as controls for both analysis (ELISA and qRT-PCR). C: Macrophages were infected with M.F. and the supernatants were tested for MCP-1 production by ELISA assay. The supernatants were collected at 24 h, 48 h and 72 h following infection. Levels of MCP-1 were indicated at each time point in M.F. infected samples and in the respective controls (uninfected cells and cells treated with 243 M.F. medium). D: MCP-1 expression was analyzed by real time RT-PCR in RNA samples from macrophages infected with M.F. MCP-1 relative amount of mRNA at 18 h following the infection is shown. Macrophages treated with 243 M.F. growth media (v/V) were used as controls. MCP-1 production (ELISA) was normalized with the calcein viability assay, as described in the Materials and Methods, in both experiments. Bars denote the standard deviation. The p-values were calculated as unequal variance t-test of Mycoplasma infected cells relative to 243 media control: *p ≤ 0.05; **p ≤ 0.005. The histograms shown are representative of data from three different experiments.

Back to article page