Figure 4

EGFR protein expression and EGFR amplification in tumor cells captured by On-ship Sort. (A, B) EGFR protein expression detected by western blot (A), correlated with EGFR protein expression captured by On-chip Sort (B), for A549 low EGFR expression and no EGFR amplification, H292 (moderate EGFR expression and amplification rate), A431 (strong EGFR expression and amplification rate), and HCC827 (strong EGFR expression and large amplification rate) cell lines. (C) Comparison of EGFR amplification rates determined by qPCR with gDNA (black bar), and when usingAmpli 1 WGA products (gray bars) including WGA of captured tumor cells (blue bars).