Figure 3

Head-to-head comparison of On-chip Sort with the CellSearch profile kit. Three tumor cell lines (H1975, A549 and H1755) expressing varying levels of EpCAM were spiked into blood from a healthy donor (10 cells/mL). The samples were divided into three and then processed using our On-chip Sort protocol as well as the CellSearch profile kit in parallel. Captured tumor cells were subjected to whole genome amplification followed by mutation detection with both pyrosequencing and deep sequencing to detect specific mutations in each tumor cell. Variant frequencies detected in the controls (gDNA and WBCs from a healthy donor) and experimental samples using pyrosequencing (A) or deep sequencing (B) are graphically represented. The line represents the lower limit of detection of each method (10% for pyrosequencing and 1% for deep sequencing). Samples with 0% variant frequency were assigned a value of 0.1 for plotting purposes. Data shown here are representative of two independent experiments for each assay.