Figure 2

Performance of On-chip Sort in a spike-in experiment. (A) Histograms of EpCAM expression in H1975, A549, and H1755 non-small cell lung tumor cell lines. Fluorescence histograms of the isotype control (gray) and of the EpCAM antibody (red). (B) The mean percent detection, capture and purity of 5, 13, and 25 tumor cells isolated following spiking into 4 mL of normal blood (n = 3). The number of detected tumor cells was calculated using Flowjo software. The number of captured tumor cells was counted as the number of cells found in the collection reservoir. Purity was calculated as the number of captured tumor cells divided by the number of captured tumor cells plus white blood cells counted in the collection reservoir. (C) Linearity of tumor cell detection (blue lines) and capture (red lines) in spiking experiments. Error bars represent S.D. of the mean. Data shown here are representative of two independent experiments for each assay.