Figure 1From: A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood Cell sorter instrument and gating mechanism for tumor cells. (A) Image of the On-chip Sort cell sorter. (B) Image of the disposable microfluidic chip used. The chip is self-contained with both inlet and outlet sample/sheath reservoirs, which ensures that no fluids come into contact with the device. The target cells are separated to the collection reservoir by this shift of flow alone. A magnified view of the micro flow paths is shown in the inset on the right. (C) Sorting gates for spiked tumor cells. Tumor cells gated as cytokeratin + and/or vimentin+/CD45- were sorted. Nuclei, cytokeratin, vimentin and CD45 staining was used to distinguish tumor cells (Tumor cell, TC; nuclei+/CK+ and/or vimentin+/CD45-) from the white blood cell population (White blood cell, WBC; nuclei+/CK-/vimentin+/CD45+).Back to article page