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Figure 1 | Journal of Translational Medicine

Figure 1

From: A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood

Figure 1

Cell sorter instrument and gating mechanism for tumor cells. (A) Image of the On-chip Sort cell sorter. (B) Image of the disposable microfluidic chip used. The chip is self-contained with both inlet and outlet sample/sheath reservoirs, which ensures that no fluids come into contact with the device. The target cells are separated to the collection reservoir by this shift of flow alone. A magnified view of the micro flow paths is shown in the inset on the right. (C) Sorting gates for spiked tumor cells. Tumor cells gated as cytokeratin + and/or vimentin+/CD45- were sorted. Nuclei, cytokeratin, vimentin and CD45 staining was used to distinguish tumor cells (Tumor cell, TC; nuclei+/CK+ and/or vimentin+/CD45-) from the white blood cell population (White blood cell, WBC; nuclei+/CK-/vimentin+/CD45+).

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