Lactate dehydrogenase (LDH) activity and infarct size in hearts of the Series 1. The experiments were carried out on Control (n = 7) and Iso/Ade groups with 0 (n = 9), 5 (n = 9), and 10 (n = 9) min washout periods. Panel A - LDH activity in the effluent perfusate during 30 min reperfusion. Differences between Control and all three Iso/Ade groups (measured for the corresponding data points) were statistically significant (P < 0.05) at all data points. There was no significant difference in LDH activity between the Iso/Ade groups. Panel B – Infarct size. Mean ± SEM (columns) and representative images of the corresponding heart slices stained with 2,3,5-triphenyltetrazolium chloride (TTC). ** P < 0.01, *** P < 0.001 vs. Control; # P < 0.05 vs. Iso/Ade group with zero min washout period.