Figure 4

Inflammation analysis. (A) Immunohistofluorescence of remote and infarcted areas against CD3 (green) and CD25 (red) labeled lymphocytes and activated lymphocytes, respectively. Nuclei are counterstained with Hoechst 33342 in blue. At the bottom, zoomed images from the infarct core (scale bar = 50 μm). (B) Positive anti-CD3, CD25 lymphocytes and CD25/CD3 ratio per field in remote and infarct zones after surgical ligation vs. coil embolization (*P = 0.002, ^†P < 0.001, and ^‡P = 0.021). (C) Histograms comparing same inflammation data between remote and infarct zones in each animal model (^§P = 0.008, ^{| |}P = 0.001, and ^#P = 0.023).