Figure 4

The -26-exon FAK protein prevents apoptosis in tumor cells. A. Examination of the apoptosis of MCF-10A cells by flow cytometry. MCF-10A cells transfected with wild-type (a, c, and e) or -26-exon FAK (b, d, and f) were cultured in serum-free medium for 12 or 24 h and then harvested and analyzed by flow cytometry. The experiments were repeated three times. B. Statistical analysis of the apoptotic ratio of MCF-10A cells cultured in serum-free medium for 0 h, 12 h, or 24 h, as shown in A. Compared with the wild-type group, the apoptotic ratio in the -26-exon group was significantly decreased (*P < 0.05). C. A model demonstrating the roles of -26-exon FAK in the promotion of tumorigenesis.