Figure 7

Definitive endoderm (DE) marker Sox17 and Foxa2 expression level and correlation analysis. (A) IPCs with or without laminin 411 group mRNA level of Foxa2 was determined using RT-PCR and expressed relative to an undifferentiated control and normalized to GAPDH. (B) mRNA level of Sox17 in IPCs with or without laminin 411 was determined using RT-PCR and expressed relative to an undifferentiated control and normalized to GAPDH. (C) Correlation of insulin secretion with Foxa2 gene expression (r = 0.9531, n = 3, P < 0.05). (D) Correlation of insulin secretion with Sox17 gene expression (r = 0.7989, n = 3, P > 0.05). (E) Correlation of insulin secretion with Pdx1 gene expression (r = 0.8890, n = 3, P < 0.05). Bars: mean ± SEM (n = 3), and *P < 0.05.