Illustrations of the IPC differentiation protocol. Undifferentiated cells were suspended in complete culture media and aliquots of 2.5 × 105 cells and placed in 6-well plates overnight. The medium was replaced with complete culture media either with (control) or without pre-coated 5 μg/mL laminin 411 and 25 mM glucose for 3 d (stage I). At stage II, the media were refreshed with DMEM/F-12 medium containing Insulin-Transferin-Selenium-A for another 4 d. At stage III, 10 mM nicotinamide was added to the media, and culture lasted for 3 days. At stage IV, the medium was changed with the same supplements at stage III, but N2 and B27 supplements were added and incubated for 4 d.