Effects on cell proliferation, migration, invasion and MMP -9/ MMP- 2 activities of HO- 8910 PM cells after transfection with FOXM1 shRNA. HO-8910 PM cells were either untransfected (Mock), or transfected with nonspecific shRNA (control) or FOXM1 shRNA (shRNA). A Cell proliferation rates were determined by MTT assay. B HO-8910 PM cells were incubated with serum-free media for 24 h and conditioned media were collected and subjected to gelatin zymography. Zymographic analysis showed decreased MMP-2 and MMP-9 activity in HO-8910 PM cells transfected with FOXM1 shRNA. C Wound healing assays were done to determine the effects on cell migration of HO-8910 PM cells after transfection with FOXM1 shRNA. The wound gaps were measured at six reference points along the wound, and the results were expressed as the average wound gap. Scale bars = 0.5 mm. D-E HO-8910 PM cells were transfected with mock, control or FOXM1 shRNA and then subject to transwell migration/invasion assays, as described in Methods. After 24/48 h, migratory/invasive cells were counted after staining with crystal violet. Scale bars = 0.1 mm. Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, statistically significant difference.