Effects on MMP- 2, MMP- 9 and VEGF- A expressions of HO- 8910 PM cells after transfection with FOXM1 shRNA. HO-8910 PM cells were either untransfected (Mock), or transfected with nonspecific shRNA (control) or FOXM1 shRNA (shRNA). A Western blot demonstrated FOXM1, MMP-2, MMP-9 and VEGF-A protein levels were significantly reduced after transfection with FOXM1 shRNA. The tubulin was analyzed as a control. B-E FOXM1, MMP-2, MMP-9 and VEGF-A mRNA expression levels of HO-8910 PM cells after transfection with FOXM1 shRNA were quantified by real-time PCR using GAPDH as a reference. Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, statistically significant difference.