Effects on cell proliferation, migration, invasion and MMP -9/ MMP- 2 activities of HO -8910 cells after transfection with FOXM1. HO-8910 cells were either untransfected (Mock), or transfected with pcDNA3.1 (control) or pcDNA3.1-FOXM1 (FOXM1). A Cell proliferation rates were determined by MTT assay. B HO-8910 cells were incubated with serum-free media for 24 h and conditioned media were collected and subjected to gelatin zymography. Zymographic analysis showed increased MMP-2 and MMP-9 activity in FOXM1-transfected HO-8910 cells. C Wound healing assays were done to determine the effects on cell migration of HO-8910 cells after transfection with FOXM1. The wound gaps were measured at six reference points along the wound, and the results were expressed as the average wound gap. Scale bars = 0.5 mm. D-E HO-8910 cells were transfected with mock, control or FOXM1 and then subject to transwell migration/invasion assays, as described in Methods. After 24/48 h, migratory/invasive cells were counted after staining with crystal violet. Scale bars = 0.1 mm. Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, statistically significant difference.