Effects on MMP-2, MMP-9 and VEGF-A expressions of HO- 8910 cells after transfection with FOXM1. HO-8910 cells were either untransfected (Mock), or transfected with pcDNA3.1 (control) or pcDNA3.1-FOXM1 (FOXM1). A Western blot demonstrated FOXM1, MMP-2, MMP-9 and VEGF-A protein levels were significantly increased after transfection with FOXM1. The tubulin was analyzed as a control. B-E FOXM1, MMP-2, MMP-9 and VEGF-A mRNA expression levels of HO-8910 cells after transfection with FOXM1 were quantified by real-time PCR using GAPDH as a reference. Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, statistically significant difference.