Figure 1

Analysis of cytotoxicity in human villous explants. Villous explant supernatants were collected and lactate dehydrogenase (LDH) activity was measured using the LDH Liquiform kit. The positive control was established by adding 0.2% Triton X-100 (A). Human villous explants were infected or not by T. gondii and treated with increasing concentrations of azithromycin or PSA for 24 h and the percentage of tissue toxicity was analyzed (B). Data are expressed as mean ± SEM. Significant differences were analyzed by ANOVA and Bonferroni multiple comparison post hoc test. ^***P < 0.001 in relation to the positive control; ^ΦΦ P < 0.01 between uninfected and infected villous explants.