Intratumoral distribution of EGFR mutations and copy number in metastatic lung cancer, what impact on the initial molecular diagnosis?

Table 2 Multi-localization EGFR genotyping screening

	Number and type of samples analyzed per patients					EGFR genotyping results
Patients	Mutation type	Primary tumor(sugical sample,lung biopsy*,bronchial biospy£)	PLEURAL BIOPSY	LYMPH NODE	Cytology: Pleural Effusion,Bronchial aspiration*	Contributive samples	Concordant EGFR status	Non contributive samples	Discordant EGFR status
P1	del19	7	0	0	0	7	7	0
P2	del19	2	0	0	0	2	2	0
P3	del19	0	3	0	1	4	3	0	1 (rescued by CAST PCR)
P4	del19	0	4	0	1	5	5	0
P5	del19	0	3	0	1	4	4	0
P6	del19	1* + 1*	0	0	0	2	2	0
P7	del19	0	0	0	2	2	2	0
P8	del19	1* + 1*	0	0	0	2	2	0
P9	del19	3	1	0	0	4	4	0
P10	del19	0	3	0	0	3	3	0
P11	del19	0	3	0	0	3	3	0
P12	del19	0	3	0	0	2	2	1
P13	del19	4	0	0	0	4	4	0
P14	del19	2	0	0	0	2	2	0
P15	del19	0	2	0	1	3	3	0
P16	del19	1£ + 1£	0	0	1*	3	1	0	2 (remained WT by CAST PCR)
P17	del19	3	0	0	0	3	3	0
P18	del19	0	0	1	1*	1	1	1
P19	del19	1 + 1*	0	0	0	2	2	0
P20	p.G719A	1	0	4	0	5	5	0
P21	p.G719A	5	0	2	0	7	7	0
P22	ins20	3	0	1	0	4	4	0
P23	ins20	0	2	0	0	2	2	0
P24	ins20	3	0	2	0	4	4	1
P25	ins20	0	2	0	0	2	2	0
P26	ins20	3	0	0	0	3	3	0
P27	ins20	2 + 1	0	1 + 1	0	4	2	1	2
P28	p.L858R	4	0	2	0	6	6	0
P29	p.L858R	1£ + 1£	0	0	0	2	2	0
P30	p.L858R	2	0	0	0	2	2	0
P31	p.L858R	3	0	0	0	3	3	0
P32	p.L858R	0	3	0	1	4	4	0
P33	p.L858R	0	3	0	0	3	3	0
P34	p.L858R	1£	1	0	1	3	3	0
P35	p.L858R	4	0	2	0	6	6	0
P36	p.L858R	2	0	1	0	3	3	0
P37	p.L858R	3	0	0	0	3	3	0
P38	p.L858R	3	0	0	0	3	3	0
P39	p.L858R	3	0	0	0	3	3	0
P40	p.L861Q/p.T790M	19	0	0	0	19	19	0

Shows the type and number of samples tested for EGFR mutation in 40 patients. Non-contributive samples are non-amplified specimens. Discordant samples are DNAs that have been found wild type when a mutation was expected. The discordant samples are italic. Lung biopsy: *and bronchial biopsy: £.

ISSN: 1479-5876