Figure 9

PI3K/Akt/GSK-3β and ERK1/2 signals are involved in the protective effect of bFGF both in SCI rats and PC12 cells under stress. A. The protein expressions of p-Akt/Akt, p-ERK/ERK, p-GSK-3β/GSK-3β in the sham, SCI model and SCI rat treated with bFGF groups. B. The optical density analysis of p-Akt/Akt, p-ERK/ERK, p-GSK-3β/GSK-3β protein. ** P < 0.01 versus the sham group, and ^# P < 0.05 versus the SCI group. Data are the mean values ± SEM, n = 6. C. MTT results of the different concentrations of TG-treated PC12 cells. D. MTT result of NGF-treated PC12 cells induced by TG. E. FACScan result of PI/Annexin V-FITC staining for cell apoptosis analysis. F. Statistical result of apoptosis rate in PC12 cells treated with TG and NGF. ** P < 0.01 versus the control group, and ^# P < 0.05 versus the TG group. Data are the mean values ± SEM, n = 3. G. The protein expressions of CHOP, GRP78, p-Akt, p-ERK1/2, p-GSK-3β in ER stress-induced apoptosis PC12 cells treated with NGF and different inhibitors. GAPDH was used as the loading control and for band density normalization. H. The optical density analysis of CHOP, GRP78, p-Akt, p-ERK1/2 and p-GSK-3β protein. * P < 0.05 versus the control group, ^# represents P < 0.05 versus the TG group, ^$ P < 0.05 versus the TG + NGF group. Data are the mean values ± SEM, n = 6.