NGF treatment increases the level of GAP43 in spinal cord lesions. A. Immunofluorescence staining results of GAP43; the nuclear is labeled by Hoechst (blue), the neurons with obvious GAP43 signals are labeled by bright right dots, magnification was 20 ×. B. The protein expressions of GAP43 in sham, SCI rats and SCI rats treated with NGF groups. GAPDH was used as the loading control and for band density normalization. C. The optical density analysis of GAP43 protein. ** P < 0.01 versus the SCI group, and ## represents P < 0.01 versus the sham group. Data are the mean values ± SEM, n = 6.