Figure 6
NGF administration inhibits the expressions of ER stress-induced apoptosis response proteins, GRP78, CHOP and caspase-12. A. Immunohistochemistry for GRP78, CHOP and caspase-12 in the sham, 7d and 14d after spinal cord injury lesion and NGF treatment 7 d and 14d after injury groups. B. Analysis of the positive cells and C. optical density of the immunohistochemistry results. ** P < 0.01 versus the SCI (7d) group, # represents P < 0.05 versus the SCI(14d) group, Data are the mean values ± SEM, n = 6. D. Protein expressions of GRP78, CHOP and caspase-12 for the sham, SCI and NGF treatment groups. GAPDH was used as the loading control and for band density normalization. E. The optical density analysis of GRP78, CHOP, and caspase-12 protein. ** P < 0.01 versus the sham group. Data are the mean values ± SEM, n = 6.