Assessment of SCI model rats. A. HE staining results of SCI rat at 1, 3, 7 and 14 d after contusion. B. The BBB scores of SCI model rat at 1, 3, 7 and 14 d after contusion. The score of the sham group was 21 points (meaning normal locomotion). ** P < 0.01 versus the sham group. Data are the mean values ± SEM, n = 6. C. TUNEL apoptosis assay of model rat spinal cord lesion. Immunofluorescence results of the TUNEL assay. Bright green dots were deemed positive apoptosis cell. Motor function was evaluated by Nissl staining. Big, blue dots were deemed as motor neuron. Magnification was 20 ×. D. The analysis of apoptosis cell 1, 3, 7 and 14 d after spinal cord injury lesions. The percentage of apoptosis was counted from 3 random 1 × 1 mm2 areas. ** P < 0.01 versus the sham group. Data are the mean values ± SEM, n = 6.