Figure 1

Tumor associated autoantibodies were detected in the sera of TgMMTV-neu mice prior to the development of palpable disease. Antigen specific IgG (●), IgM (▲) and tumor growth (■) were identified in a single animal and evaluated over time for (A) Pdhx, (B) Otud6b, (C) Stk39, (D) Lgals8, (E) Vps35 and (F) Znf238. IgG (○) and IgM (∆) measured over time from control animals (n = 20 (+/−SE for first and last time points and for an individual control for all intermediate time points). Left Y axis: Tumor volume; right Y axis: antibody concentration or band density; X axis: mouse age (in weeks). Arrow indicates first palpable tumor. * indicates p < 0.05 from initial value.