Figure 3
From: Apoptotic function of tumor-associated antigen RCAS1 in oral squamous cell carcinoma
Detection of soluble RCAS1 in OSCC cell lines using dot blotting and ELISA. A Analyses of dot blotting revealed soluble RCAS1 in culture supernatants in all OSCC cell lines, which increased according to culture period. B ELISA analyses revealed significantly more soluble RCAS1 in the culture supernatant of SQUU-B than in the other 3 OSCC cell lines at day 4 (P < 0.05; Mann–Whitney U-test). Mean concentrations of quadruplicate measurements are shown.