Figure 6

SATB2 was a tumor- suppressor in CRC and attenuated miR- 182- mediated malignant phenotype in CRC. (A) Cell proliferation was measured by CCK-8 assay to compare the growth difference between SW480 cells with over-expression of STAB2 and SW480 mock cells with transfection of empty vector. (B, C) The invasiveness and migration ability were detected by matrigel invasion assay (B) and wound-healing assay (C) in SW480 cell after SATB2 over-expression. Cell proliferation (D), invasion (E) and migration (F) changes were detected between SW480/miR-182 cells and SW480/miR-182 cells with over-expression of STAB2. SATB2 could inhibit cell growth, invasion and migration of SW480/miR-182. SW480/miR-182 denoted CRC SW480 cells with stable over-expression of miR-182. * p < 0.05, ** p <0.001.