Figure 6

Effects of SYD on EMT-related protein expression. (A) Immunohistochemical staining of E-cadherin, N-cadherin, fibronectin, vimentin in tumor tissues or normal colonic tissues. 200 × for all, scale bar = 100 μm. (B) Western blot of E-cadherin, N-cadherin, vimentin, Snail, and claudin-1 in colonic tissues. E-cadherin was significantly deficient in the model group. E-cadherin was upregulated while N-cadherin, fibronectin, and vimentin were downregulated by SYD treatment. These effects were confirmed by Western blot. Semiquantitive analysis of these proteins is showed in Table 4.