Figure 5

Silencing of XB130 modulated phosphorylation of Akt and expression of EMT-associated proteins by cultured GC cell lines. (A) Phosphorylation of Akt was significantly decreased by XB130 knockdown. (B) Immunostaining for E-cadherin and vimentin. E-cadherin was significantly upregulated and vimentin was downregulated by XB130 knockdown. Scale bar = 50 μm. n = 3 in each group. (C) Representative results of Western blotting. In comparison with the Scramble group, silencing of XB130 upregulated the expression of E-cadherin, α-catenin and β-catenin, while it downregulated expression of fibronectin, vimentin, CD44, MMP9 and MMP2. (D) Quantitative analysis of panel C. ^# p < 0.01 versus the Scramble group, n = 3. Ctrl = control, Scr = Scramble, Sh = sh-XB130.