Figure 4

XB130 silencing reduced the growth of xenograft tumors. SGC7901 cells were implanted in nude mice by subcutaneous injection into the anterior right flank, while the same amounts of wild-type, Scramble RNA-transfected, or sh-XB130-transfected SGC7901 cells were injected into the posterior left and right flanks. (A) After subcutaneous injection, tumors always formed, as shown in this representative picture taken on day 21. (B) Growth curves of the tumors. Tumor volumes were measured over 21 days in each group. n = 5 per group, *p < 0.05 and ^# p < 0.01. (C) Tumors harvested from the three groups on day 21. (D) Representative images of immunostaining for XB130, E-cadherin, vimentin, and p-Akt in xenograft tumors. Scale bar = 100 μm. n = 3 in each group.