Over-expression of ANX A1 suppressed CBDL-rat-serum-induced increase in the a1-actin, a1-tubulin and destrin protein expression levels in PMVECs. (A) The change in expression levels of a1-actin, a1-tubulin and destrin proteins in PMVECs 24 h following either mock transfection or transfection with Ad-ANXA1 was determined by western blot. GAPDH was used as an internal control. (B-D) The protein density of a1-actin, a1-tubulin and destrin for each group is displayed. Each data point represents the mean ± S.E.M. of four independent experiments. * p < 0.05 vs. group N; #p < 0.05 vs. the non-transfected group; Δp < 0.05 vs. T1. N: Non-transfected; T: transfected with Ad-ANXA1.