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Figure 2 | Journal of Translational Medicine

Figure 2

From: Intra-osseous injection of donor mesenchymal stem cell (MSC) into the bone marrow in living donor kidney transplantation; a pilot study

Figure 2

Donor-Specific Lymphocyte Proliferation (mixed lymphocyte reaction). Heparinized blood sample of recipient was collected before (day-35) and after (day + 30) the MSC-kidney transplantation. The recipients lymphocytes (responder) were co-cultured with irradiated donor lymphocytes (stimulator) for 4 days. Donor-specific recipients’ lymphocyte proliferation was determined by 3H-thymidine incorporation using a liquid scintillation counter (Beckman LS 6500; Beckman Instruments Inc., Fullerton, CA, USA). Proliferative response was represented by DPM. Plus signs indicate statistically significant induction of proliferative response (p < 0.05) compared to D-35 baseline activity. Asterisks indicate the statistical significance of decreased proliferative response compared to D-35 baseline activity (p < 0.05).

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