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Figure 3 | Journal of Translational Medicine

Figure 3

From: Preferential transfer of mitochondria from endothelial to cancer cells through tunneling nanotubes modulates chemoresistance

Figure 3

Intercellular transfer of cytoplasmic contents through TnTs. Tumoral cells were co-cultured with eGFP-E4+ECs or M-Orange-MSCs. Before imaging by confocal microscopy, the co-cultures were stained with WGA AF594 to reveal cell membranes. Confocal imaging showed hetero-cellular TnTs containing GFP (A) or M-Orange (B). WGA-stained cytoplasmic content were also seen at several points in TNTs (C, arrows). Subsequently GFP or M-Orange could be observed in recipient cancer cells in different panels. A, GFP dots (arrows) could be observed in recipient cells in different panels (MCF7 on the top, MDA on the center, SKOV3 on the bottom) Scale bar: 10 μm. B, M-orange could be detected in recipient OVCAR3 cancer cells. Scale bar: 20 μm. C, Scale bar: 10 μm. D, detail frames extracted from time-lapse recording of the movie 2. This event happens at 55 seconds in the left side of movie 2. Duration was reported, 34 min 53 second in total. Cytoplasmic material was transferred through TnTs as indicated (arrow). Scale bar: 10 μm.

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