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Figure 2 | Journal of Translational Medicine

Figure 2

From: Preferential transfer of mitochondria from endothelial to cancer cells through tunneling nanotubes modulates chemoresistance

Figure 2

Time-lapse studies of TnTs formation. A, detailed frames extracted from time-lapse recording of the movie 2. This event happened at 43 seconds in the left corner of movie 2. Duration was reported, 38 min 42 second in total. This set of pictures allows us to see formation of a TnT by membrane projection between MCF-7 and MSCs co-cultured for 24 hours. Scale bar: 10 μm. B-C, detailed frames extracted from time-lapse recording of the movie 2. This event happened at 1 min 10 seconds in the left top corner (B) and at 1 min 12 seconds on the right top corner (C). Duration was reported, 49 min 32 seconds (B) and 2 hours 42 min (C). Serial images showing the formation of a nanotube following large membrane adhesion between MCF-7 and MSCs Scale bar: 20 μm. D, Detailed frames extracted from time-lapse recording of the movie 5. MCF-7 and eGFP-E4+ECs were co-cultured and stained using WGA AF594 to reveal cell membranes. Separated cells migrated toward each other. After the contact lasting the two cells formed nanotubes (arrows) elongating as the cells separated further. Scale bar: 10 μm.

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