Effects on cellular invasiveness/migration and MMP-9/MMP-2 expressions of HO-8910PM cells after transfection with basigin-2 siRNA. HO-8910PM cells were either untransfected (blank), or transfected with nonspecific siRNA (control) or basigin-2 siRNA (siRNA). A-B Western blot demonstrated basigin-2 protein levels were significantly inhibited by basigin-2 siRNA. C-D HO-8910PM cells were incubated for 24 h and conditioned media were used for the measurement of MMP-2 and MMP-9 protein levels by gelatin zymography. Zymographic analysis showed decreased MMP-2 and MMP-9 activity in HO-8910PM cells transfected with basigin-2 siRNA. E-F Wound healing assay was performed on monolayers of HO-8910PM cells transfected with basigin-2 siRNA. The wound gaps were measured at six reference points along the wound, and the results were expressed as the average wound gap. G-H Suppression of basigin-2 expression in HO-8910PM cells resulted in significant reduction in cellular migration. I-J Suppression of basigin-2 expression in HO-8910PM cells resulted in significant reduction in cellular invasiveness. Scale bars = 0.5 mm (E). Scale bars = 0.1 mm (G, I). Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, statistically significant difference.