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Figure 2 | Journal of Translational Medicine

Figure 2

From: Sequencing CTLA-4 blockade with cell-based immunotherapy for prostate cancer

Figure 2

Time-dependent augmentation of anti-tumor CD8 T cell response by the addition of CTLA-4 blockade to GVAX immunotherapy. A: Timing of GVAX immunotherapy and CTLA-4 blockade in non-transgenic mice. Top panel shows experimental design. Readout was HA-specific lytic function quantified by an in vivo CTL analysis. Endogenous T cell lytic function was assayed; no adoptive T cell transfer was performed. 5 animals per group, representative of 2 experiments. B: Timing of GVAX immunotherapy and CTLA-4 blockade in tumor-bearing, ProHA x TRAMP mice. Top panel: experimental design. Bottom panel: CTL function in ProHA x TRAMP mice. As in Figure 2A these studies were performed without adoptive T cell transfer, i.e. endogenous T cell lytic activity was assayed. 5 animals per group, representative of 2 experiments. C: Effects of more frequent anti-CTLA-4 administration. Experimental design (top panel) is identical to Figure 2B, with the exception of more frequent anti-CTLA-4 administration. 5 animals per group, representative of 2 experiments. D: Day 0 vs. Day 1 anti-CTLA4 dosing. Experimental design (top panel). Dosing of anti-CTLA-4 on Day 1 was directly compared with same day (Day 0) GVAX/anti-CTLA-4 dosing. 5 animals per group, representative of 2 experiments.

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