Figure 1

Procedure for CIK preparation and infusion. Peripheral blood mononuclear cells (PBMC) are isolated by apheresis. T cells are activated, expanded, and differentiated by anti-CD3 in the presence of cytokines including IFN-γ, IL-1α, and IL-2 for 14 to 21 days. These T cells, commonly called CIK, are then infused into patients.