Figure 3

Analysis of cell susceptibility to GLV-1h68 cytotoxicity in HCT-15 and HCT-116 CRC cells. A) Plaque forming ability in CV-1 cells (standard), HCT-15 (low cytotoxicity), and HCT-116 (high cytotoxicity). All cell lines were tested in triplicates with a dose of 1x10⁸ pfu of GLV-1h68 for 24 hours at 37°C. Cells were then harvested and plaque forming ability was determined by standard plaque assay. Average titers were determined from triplicates and expressed as pfu/mL. Averages plus standard deviations were plotted. B) Cell proliferation of HCT-15 and HCT-116. Cells were seeded at a concentration of 1x10³ cells/well and cell proliferation was followed for 24, 48, 72, 96 and 120 hours. Cell lines were tested in quadruplicates and averages plus standard deviations are plotted. C) Replication of GLV-1h68 at low and high MOIs. Cells were infected at an MOI of 0.01 (low) or 10 (high) and samples were collected at 6, 24, and 48 hours after infection. Average titers were determined in from triplicate and normalized to pfu/1x10⁶ cells. Averages plus standard deviations are plotted.