Figure 2

Enhanced hepatocyte survival after co-culture with MSCs + IL-6. A) Gene expression of apoptotic (Bax, caspase-3, NF-κB, TNF-α) and anti-apoptotic (Bcl-xl) markers in hepatocytes co-cultured with MSCs together with IL-6. B) Gel band quantification of gene expressions through Image J (n=3). CCl₄ vs MSCs + IL-6 ^ɸ p< 0.05, ^{ɸ ɸ} p< 0.01 and ^{ɸ ɸ ɸ} p< 0.001. C) LDH (%) release levels in control and treatment groups (n=4). D) Analysis of apoptotic cells through annexin V staining (n=4). CCl₄ vs IL-6 *p< 0.05, **p< 0.01 and ***p< 0.001, CCl₄ vs MSCs ^#p< 0.05, ^##p< 0.01 and ^###p< 0.001, CCl₄ vs MSCs + IL-6 ^ɸ p< 0.05, ^{ɸ ɸ} p< 0.01 and ^{ɸ ɸ ɸ} p< 0.001, IL-6 vs MSCs + IL-6 ^ψp< 0.05, ^ψψp< 0.01 and ^ψψψp< 0.001, MSCs vs MSCs + IL-6 ^δp< 0.05, ^δδp< 0.01 and ^δδδp< 0.001.