Figure 2

Expression of granule-associated genes. (A) Mean expression level for samples with high CD177 expression (> 256) versus that for samples with low CD177 expression (< 32), for each of the 9697 probe sets passing the low-expression filter. Each point represents one probe set. Blue squares highlight CD177 and 19 probe sets for 13 unique neutrophil granule-associated genes. Diagonal reference lines indicate mean expression ratios of 4, 2, 1, 0.5, and 0.25. Expression of genes with points above the central dashed line was positively associated with CD177 expression. (B) Mean gene expression (anti-log RMA-normalized value) of CEACAM1 (probe set: 206576_s_at) during ipilimumab treatment in patients with (GI irAE) or without (No-GI irAE) a grade 2 or greater GI irAE.