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Table 2 Effect of siRNA-induced tumor IL-6 silencing on hypothalamic CRH expression and circulating levels of IL-6 and ACTH in B16-F10 tumor-bearing mice

From: Stress hormones promote growth of B16-F10 melanoma metastases: an interleukin 6- and glutathione-dependent mechanism

 

Lung metastases

 

B16-F10

B16-F10/IL-6-siRNA

Tumor IL-6 expression (-fold change)

1.0 ± 0.2

0.15 ± 0.05*

Serum IL-6 (pg/ml)

347 ± 75

132 ± 34*

Hypothalamic CRH expression (AU)

40 ± 12

85 ± 16*

Plasma ACTH (pg/ml)

240 ± 50

255 ± 63

  1. Gene transfections were performed as explained under Materials and methods. CRH expression data are expressed as optical density arbitrary units (AU). All measurements were performed 7 days after tumor cell inoculation. The data show mean values ± S.D. for 5–6 different experiments. *P< 0.01 comparing B16-F10/IL-6-siRNA-bearing mice versus control B16-F10-bearing mice.