Stress hormones promote growth of B16-F10 melanoma metastases: an interleukin 6- and glutathione-dependent mechanism

Table 1 Effect of corticosterone and NORA on IL-6 expression and secretion by B16-F10 and B16-F10-RFP cells in vitro

Tumor cells	Additions	Expression (-fold change)	Secretion (pg/10⁶cells x 24 h)
B16-F10	None	1.0 ± 0.1	833 ± 170
	Corticosterone	2.5 ± 0.4^*	1654 ± 266^*
	NORA	1.7 ± 0.2^*	1257 ± 214^*
	Corticosterone+NORA	2.4 ± 0.3^*	1712 ± 247^*
B16-F10-RFP	None	3.0 ± 0.3^*+	1966 ± 331^*+
	Corticosterone	3.3 ± 0.4^*+	2184 ± 287^*+
	NORA	3.0 ± 0.3^*+	1763 ± 294^*+
	Corticosterone+NORA	3.6 ± 0.5^*+	2477 ± 315^*+

IL-6 expression and release to the extracellular medium were measured (48 and 72 h after seeding, respectively) as indicated under Materials and methods. Corticosterone and/or NORA were incubated (at the concentrations indicated in the text) 24 h after seeding and were present in the incubation medium for 6 h, then the medium was renewed. Data are mean values + S.D. (n= 5-6) calculated for the indicated period. *P< 0.01 comparing both cell types and all conditions versus control B16-F10 cells; +P< 0.01 comparing all additions under B16-F10-FRP cells versus their equivalents under B16-F10 cells.

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