Figure 4

miRNA-15b regulates phenotypes of CD8+ T cell but does not affect proliferation of T cell. A) After transfected with mimic-15b or mimic-NC, CD8+ T cells were co-stimulated by CD3/CD28 mAb. The expression of CD69 before or 48 h after co-stimulation was detected by flow cytometry analysis. The red line represented an isotype-matched control. The blue line represented the staining of CD69 mAb conjugated with APC. B) The expression of CD44 before or 120 h after co-stimulation was detected by flow cytometry analysis. The red line represented an isotype-matched control. The blue line represented the staining of CD44 mAb conjugated with PE. C) Before and after co-stimulation by CD3/CD28 mAb, the positive rates of CD69 (left) or CD44 (right) in CD8+ T cells with mimic-15b or mimic-NC were evaluated. The data were obtained from three independent experiments (mean ± standard deviation), *P < 0.01. D) CFSE-dilution analysis on CD8+ T cells transfected with mimic-15b or mimic-NC after 96 h of co-stimulation with CD3/CD28 mAb. Red line represented T cells with mimic-15b and blue line represented T cells with mimic-NC.