Figure 2

Dynamic expression of miRNA-15b in CD8+ T cells was detected by qRT-PCR. A) The relative expression of miRNA-15b in CD8+ Te and Tm cells was detected by qRT-PCR. U6 was used as an internal control to normalize the relative amounts of miRNA-15b. *P < 0.01. B) Relative expression of miRNA-15b was detected in CD8+ T cells from tumor-burdened mice or healthy ones by qRT-PCR. The results are presented as mean ± standard deviation and obtained from 6 independent experiments (6 tumor-burdened mice vs 6 healthy mice). *P < 0.05. C) CD8+ T Cells were co-stimulated by CD3/CD28 mAb and the levels of miRNA-15b were measured at different time points by qRT-PCR.