Figure 2

Discrepancy between SLC22A18 promoter methylation and protein expression. Representative samples for SLC22A18 promoter methylation and protein expression (1, 2) as well as lack of promoter methylation and protein expression (3, 4) are shown. (A) Polyacrylamide gel showing amplification products of unmethylated and methylated DNA sequences by MSP. Positive controls for unmethylated (pcu) and methylated (pcm) sequences were shown. (B) Western blot analysis for the corresponding cases.