Figure 3

Analysis of CD4 and CD8 T cell subsets, immunosenescence and exhaustion. Panel A. Fresh blood was stained with the antibody combinations described in Table 1. Different CD4 and CD8 T cell subpopulations (Naïve, Central memory, Transitional memory and Effector memory) were identified by CD27, CD27 and then CCR7 and CD45RA expression as shown. Panel B. The median values for the frequency of the indicated subsets in healthy donors and CFS affected individuals are shown in circular plots. Significant differences among groups are indicated. Panel C. The entire CD4 and CD8 T cell gates were also analyzed for the expression of CD57, PD-1 and Fas-CD95. In all cases, data from healthy donors (n = 25, HD) and SFC affected individuals (n = 19, SFC) are shown, with median (thick lines), interquartile range (boxes) and 10–90 percentile values (bars). In all cases, p-values for nonparametric Mann–Whitney comparison are shown.