A, CXCL14 expression was elevated in human colorectal cancer. Individual FFPE sections (e.g. case 1) demonstrated normal colorectal mucosa, with adjacent colorectal cancer that had invaded underneath the normal mucosa. Inset in the left panel, the junction between normal mucosa and adjacent tumor, shown at a higher magnification in the right panel, demonstrated the differences in the level of CXCL14 expression. ‘T’ refers to tumor tissue and ‘N’ refers to adjacent non-tumor tissue from the same patient. Bar, 100 μm. Paired samples of normal colorectal tissue and colorectal tumor tissue from the same patients (e.g. case 2) demonstrated little detectable CXCL14 immunoreactivity in the normal colorectal mucosa. In contrast, the colorectal tumor demonstrated intense CXCL14 immunostaining in the cytoplasm of the tumor. Bar, 50 μm. B, CXCL14 expression was plotted using the immunohistochemical scores in each carcinoma and adjacent normal tissues. C, CXCL14 expression scores were shown as box plots, with the horizontal lines representing the median; the bottom and top of the boxes representing the 25th and 75th percentiles, respectively; and the vertical bars representing the range of data.