Figure 4
From: Anti-KIT designer T cells for the treatment of gastrointestinal stromal tumor
Ability of human anti-KIT dTc to effectively lyse KIT+ tumor cells. (A) LDH assay to evaluate enzymatic release following tumor cell lysis was performed on supernatant from co-culturing of 1st and 2nd generation dTc with irradiated GIST 882 cells. Maximal release was defined by the highest experimental values. (B-C) To confirm the cytotoxic ability of dTc, irradiated GIST 882 or GIST 48 cells were labeled with CFSE and cultured with unlabelled T cells. Tumor cell death was quantified by measuring the decrease in CFSE fluorescence by gating on remaining live cells. Data are representative of three or more repetitions.