Figure 2
From: Anti-KIT designer T cells for the treatment of gastrointestinal stromal tumor
Transduction efficiency and phenotype of human anti-KIT designer T cells (dTc). (A) PBMC were isolated and primary human T cells activated and transduced with retrovirus expressing KIT-specific CIRs. Shaded histograms represent dTc and open histograms represent untransduced cells. (B) Flow cytometric analysis of the phenotype of 1st and 2nd generation dTc demonstrated that T cells (CD4+ and CD8+ T cells) of a central memory phenotype (CD45RO+CD62L+CCR7+) were in the majority. Naïve T cells were defined as (CD45RO-CD62L+). Data are representative of three or more repetitions.